Journal: Scientific reports

Article Title: Administration of an antibody against apoptosis inhibitor of macrophage prevents aortic aneurysm progression in mice.

doi: 10.1038/s41598-024-66791-7

Figure Lengend Snippet: Figure 5. AIM distribution. (A,B) Representative images of immunofluorescence staining for AIM in the IgG and AIM groups. AIM (red) and cell nuclei (blue). Scale bars = 50 μm. (C) The ratio of the AIM-positive area to the DAPI-positive area, expressed as a percentage. Data are means ± SEM. **p < 0.01 assessed by the Mann– Whitney U test. The “#” symbols enclosed by dashed lines indicate the vessel lumens.

Article Snippet: In all slides, cell nuclei were stained with DAPI Fluoromount-G (Southern Biotech, Birmingham, AL, USA).

Techniques: Immunofluorescence, Staining, MANN-WHITNEY

Journal: Scientific reports

Article Title: Administration of an antibody against apoptosis inhibitor of macrophage prevents aortic aneurysm progression in mice.

doi: 10.1038/s41598-024-66791-7

Figure Lengend Snippet: Figure 6. Macrophage polarization. (A) iNOS (red) and CD68 (green); (B) CD206 (red), CD68 (green), and cell nuclei (blue). Scale bars = 100 μm. (C) The percentage of CD68 + cells to DAPI + cells. (D,E) The percentage of the CD68 + population consisting of iNOS + /CD206 + cells. (F) The percent ratio of CD206 + cells to iNOS + cells in the CD68 + population. Data are means ± SEM. *p < 0.05 and **p < 0.01 assessed by the Mann– Whitney U test. iNOS + /CD68 + cells indicate M1 macrophages, while CD206 + /CD68 + cells indicate M2 macrophages. The “#” symbols enclosed by dashed lines indicate the vessel lumens.

Article Snippet: In all slides, cell nuclei were stained with DAPI Fluoromount-G (Southern Biotech, Birmingham, AL, USA).

Techniques: MANN-WHITNEY